Experimental Characterisation of Fluid Mechanics in a Spinner Flask Bioreactor
نویسندگان
چکیده
منابع مشابه
Experimental Characterisation of Fluid Mechanics in a Spinner Flask Bioreactor
The spinner flask bioreactor has been widely used in in vitro cell culturing processes due to its superiority in providing a homogeneous culture environment compared to traditional culturing methods. However, there is limited understanding of the flow fields in these bioreactors, and optimum culture conditions are yet to be determined. This article presents the experimental characterization of ...
متن کاملFluid Flow and Stresses on Microcarriers in Spinner Flask Bioreactors
The paper presents a validated CFD model for the flow in a bench-scale spinner flask that features a centrally-located magnet-driven impeller. Model development found that use of the immersed solid mesh method resulted in better correspondence with observations and μPIV measurements than the more established sliding mesh method in ANSYS-CFX. Analysis of spatial distributions suggested stresses ...
متن کاملComparison of the Production of Recombinant Protein in Suspension Culture of Cho Cells in Spinner Flask and Shake Flask System
Chinese hamster ovary (CHO) cells is one of the most widely used production host for the commercial production of biopharmaceuticals product. They have been extensively studied and developed, and today provide a stable platform for producing monoclonal antibodies and recombinant proteins. This study focused on the production of recombinant protein in suspension culture of CHO cells in spinner f...
متن کاملOptical flow estimation in experimental fluid mechanics
We present in this paper a novel approach dedicated to the measurement of velocity in fluid experimental flows. Such information, which is fundamental for specialists, is usually computed by correlation methods on a special kind of images (named PIV). We present here a motion estimation technique which is based on an optical-flow extension. Results are presented on an experimental flow and are ...
متن کاملFlow Characterization of a Spinner Flask for Induced Pluripotent Stem Cell Culture Application
We present detailed quantitative measurement analyses for flow in a spinner flask with spinning rates between 20 to 45 RPM, utilizing the optical velocimetry measurement technique of Particle Image Velocimetry (PIV). A partial section of the impeller was immersed in the working fluid to reduce the shear forces induced on the cells cultured on microcarriers. Higher rotational speeds improved the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Processes
سال: 2014
ISSN: 2227-9717
DOI: 10.3390/pr2040753